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                  English
                  Technical Information

                  技術資料

                  快速查找限制酶工具

                  貨號 名稱 同裂酶 異裂酶 識別位點 切割末端 反應溫度 失活條件 甲基化影響
                  EG23512S Nt.BspQI GCTCTTCN

                  5'...G C T C T T C N↓...3'
                  3'...C G A G A A G N ...5'
                  50℃ 80℃, 20 min

                  GMP501S BsaI, GMP Grade Eco31I, Bso31I, BspTNI GGTCTC(1/5)

                  5'...G G T C T C (N)? ↓...3'
                  3'...C C A G A G (N)? ↑...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: impaired.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG23514S Nb.BsrDI GCAATGNN

                  5'...G C A A T G N N...3'
                  3'...C G T T A C↑N N ...5'
                  65℃ 80℃, 20 min

                  GMP502S XbaI, GMP Grade TCTAGA

                  5'...T ↓ C T A G A...3'
                  3'...A G A T C ↑ T...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: blocked.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15502S LightNing™ AccI XmiI, FblI GTMKAC

                  5'...G T ↓ M K A C...3'
                  3'...C A K M ↑ T G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG23501S LightNing™ AgeI AsiGI, CspAI, BshTI, PinAI ACCGGT

                  5'...A ↓ C C G G T...3'
                  3'...T G G C C ↑ A...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15601S LightNing™ ApaLI Alw44I, VneI GTGCAC

                  5'...G ↓ T G C A C...3'
                  3'...C A C G T ↑ G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: impaired.
                  EcoBI: no effect.

                  EG15508S LightNing™ AscI PalAI, SgsI GGCGCGCC

                  5'...G G ↓ C G C G C C...3'
                  3'...C C G C G C ↑ G G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15509S LightNing™ AvrII AspA2I, BlnI, XmaJI CCTAGG

                  5'...C ↓ C T A G G...3'
                  3'...G G A T C ↑ C...5'
                  5’ protruding ends 37℃ No (column purification)

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15510S LightNing™ BamHI GGATCC

                  5'...G ↓ G A T C C...3'
                  3'...C C T A G ↑ G...5'
                  5’ protruding ends 37℃ No (column purification)

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15513S LightNing™ BclI FbaI, Ksp22I TGATCA

                  5'...T ↓ G A T C A...3'
                  3'...A C T A G ↑ T...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: blocked.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: some blocked.

                  EG15591S LightNing™ BglII AGATCT

                  5'...A ↓ G A T C T...3'
                  3'...T C T A G ↑ A...5'
                  5’ protruding ends 37℃ No (column purification)

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: some blocked.

                  EG15518S LightNing™ BsaI Eco31I, Bso31I, BspTNI GGTCTC(1/5)

                  5'...G G T C T C (N)? ↓...3'
                  3'...C C A G A G (N)? ↑...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: impaired.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG23505S LightNing™ BsrGI BstAUI, Bsp1407I TGTACA

                  5'...T ↓ G T A C A...3'
                  3'...A C A T G ↑ T...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15607S LightNing™ BstBI AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI TTCGAA

                  5'...T T ↓ C G A A...3'
                  3'...A A G C ↑ T T...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: some blocked.
                  EcoBI: no effect.

                  EG15528S LightNing™ BstEII BstPI,Eco91I,EcoO65I,PspEI GGTNACC

                  5'...G ↓ G T N A C C...3'
                  3'...C C A N T G ↑ G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: impaired.
                  EcoBI: impaired.

                  EG15532S LightNing™ ClaI BspDI,Bsa29I,BseCI,BshVI,Bsu15I,BsuTUI ATCGAT

                  5'...A T ↓ C G A T...3'
                  3'...T A G C ↑ T A...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: blocked.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: impaired.

                  EG15585S LightNing™ DpnI MalI Bsp143I, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI GA??TC

                  5'...G A?? ↓ T C...3'
                  3'...C T ↑ A?? G...5'
                  blunt 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: impaired.

                  EG15533S LightNing™ DpnII MboI,Sau3AI,Bsp143I, BssMI,BstMBI,Kzo9I,NdeII BstKTI, DpnI, MalI GATC

                  5'...↓ G A T C...3'
                  3'...C T A G ↑...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: blocked.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: blocked.

                  EG23502S LightNing™ DraI TTTAAA

                  5'...T T T ↓ A A A...3'
                  3'...A A A ↑ T T T ..5'
                  blunt 37℃ 80℃,20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: blocked.
                  EcoBI: no effect.

                  EG15535S LightNing™ EagI BseX3I, BstZI, EclXI, Eco52I CGGCCG

                  5'...C ↓ G G C C G...3'
                  3'...G C C G G ↑ C ..5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG23511S LightNing™ EarI Eam1104I, Bst6I CTCTTC(1/4)

                  5'...C T C T T C (N)?↓...3'
                  3'...G A G A A G (N)?↑...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15536S LightNing™ EcoRI FunII GAATTC

                  5'...G ↓ A A T T C...3'
                  3'...C T T A A ↑ G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: impaired.

                  EG15537S LightNing™ EcoRV Eco32I GATATC

                  5'...G A T ↓ A T C...3'
                  3'...C T A ↑ T A G...5'
                  blunt 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: some impaired.
                  EcoKI: no effect.
                  EcoBI: some blocked.

                  EG21503S LightNing™ Esp3I (BsmBI) BsmBI CGTCTC(1/5)

                  5'...C G T C T C (N)?↓...3'
                  3'...G C A G A G (N)?↑...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15586S LightNing™ FspI Acc16I,NsbI TGCGCA

                  5'...T G C ↓ G C A...3'
                  3'...A C G ↑ C G T...5'
                  blunt 37℃ No (column purification)

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG23509S LightNing™ HaeIII BsuRI, BshFI, BspANI, BsnI AoxI GGCC

                  5'...G G ↓ C C...3'
                  3'...C C ↑ G G...5'
                  blunt 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15539S LightNing™ HindIII AAGCTT

                  5'...A ↓ A G C T T...3'
                  3'...T T C G A ↑ A...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: some blocked.

                  EG15593S LightNing™ HinfI GANTC

                  5'...G ↓ A N T C...3'
                  3'...C T N A ↑ G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: blocked.

                  EG15541S LightNing™ HpaI KspAI GTTAAC

                  5'...G T T ↓ A A C...3'
                  3'...C A A ↑ T T G...5'
                  blunt 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: impaired.
                  EcoBI: no effect.

                  EG23507S LightNing™ HpaII MspI, HapII, BsiSI CCGG

                  5'...C ↓ C G G...3'
                  3'...G G C ↑ C...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15543S LightNing™ KasI SspDI Mly113I, EgeI, NarI, EheI, DinI, SfoI, PluTI GGCGCC

                  5'...G ↓ G C G C C...3'
                  3'...C C G C G ↑ G...5'
                  blunt 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15544S LightNing™ KpnI Asp718I, Acc65I GGTACC

                  5'...G G T A C ↓ C...3'
                  3'...C ↑ C A T G G...5'
                  3’ protruding ends 37℃ No (column purification)

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15547S LightNing™ MluI ACGCGT

                  5'...A ↓ C G C G T...3'
                  3'...T G C G C ↑ A...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: impaired.
                  EcoBI: impaired.

                  EG15548S LightNing™ MnlI CCTC(7/6)

                  5'...C C T C N? ↓...3'
                  3'...G G A G N? ↑...5'
                  3’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: blocked.

                  EG23508S LightNing™ MspI HpaII, HapII, BsiSI CCGG

                  5'...C ↓ C G G...3'
                  3'...G G C ↑ C...5'
                  5’ protruding ends 37℃ No (column purification)

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG23510S LightNing™ MunI (MfeI) MfeI CAATTG

                  5'...C ↓ A A T T G...3'
                  3'...G T T A A ↑ C...5'
                  5’ protruding ends 37℃ No (column purification)

                  EG15550S LightNing™ NcoI Bsp19I CCATGG

                  5'...C ↓ C A T G G...3'
                  3'...G G T A C ↑ C...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15551S LightNing™ NdeI FauNDI CATATG

                  5'...C A ↓ T A T G...3'
                  3'...G T A T A ↑ C...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15552S LightNing™ NheI AsuNHI BmtI, BspOI GCTAGC

                  5'...G ↓ C T A G C...3'
                  3'...C G A T C ↑ G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: some blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15553S LightNing™ NotI CciNI GCGGCCGC

                  5'...G C ↓ G G C C G C...3'
                  3'...C G C C G G ↑ C G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15602S LightNing™ NruI Bsp68I,BtuMI,RruI TCGCGA

                  5'...T C G ↓ C G A...3'
                  3'...A G C ↑ G C T...5'
                  blunt 37℃ No (column purification)

                  Dam: blocked.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15606S LightNing™ NsiI EcoT22I, Mph1103I, Zsp2I ATGCAT

                  5'...A T G C A ↓ T...3'
                  3'...T ↑ A C G T A...5'
                  3’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: some impaired.

                  EG15603S LightNing™ PacI TTAATTAA

                  5'...T T A A T ↓ T A A...3'
                  3'...A A T ↑ T A A T T...5'
                  3’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG23506S LightNing™ PmeI MssI GTTTAAAC

                  5'...G T T T ↓ A A A C...3'
                  3'...C A A A ↑ T T T G...5'
                  blunt 37℃ 80℃,20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: some blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15560S LightNing™ PspGI (EcoRII) EcoRII, Psp6I, AjnI BciT130I, BseBI, Bst2UI, BstNI, MvaI CCWGG

                  5'... ↓ C C W G G ...3'
                  3'... G G W C C ↑ ...5'
                  5’ protruding ends 75℃ No (column purification)

                  Dam: no effect.
                  Dcm: blocked.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15561S LightNing™ PstI BspMAI CTGCAG

                  5'...C T G C A ↓ G...3'
                  3'...G ↑ A C G T C...5'
                  3’ protruding ends 37℃ No (column purification)

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15604S LightNing™ PvuII CAGCTG

                  5'...C A G ↓ C T G...3'
                  3'...G T C ↑ G A C...5'
                  blunt 37℃ No (column purification)

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: impaired.

                  EG15565S LightNing™ SacI Psp124BI, SstI Ecl136II, EcoICRI, Eco53kI GAGCTC

                  5'...G A G C T ↓ C...3'
                  3'...C ↑ T C G A G...5'
                  3’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15605S LightNing™ SacII Cfr42I,KspI,Sfr303I,SgrBI CCGCGG

                  5'...C C G C ↓ G G...3'
                  3'...G G ↑ C G C C...5'
                  3’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15566S LightNing™ SalI GTCGAC

                  5'...G ↓ T C G A C...3'
                  3'...C A G C T ↑ G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: some blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15568S LightNing™ SbfI SdaI, Sse8387I CCTGCAGG

                  5'...C C T G C A ↓ G G...3'
                  3'...G G ↑ A C G T C C...5'
                  3’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15570S LightNing™ SfiI GGCCNNNNNGGCC

                  5'...G G C C N N N N ↓ N G G C C...3'
                  3'...C C G G N ↑ N N N N C C G G...5'
                  3’ protruding ends 50℃ No (column purification)

                  Dam: no effect.
                  Dcm: impaired.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15572S LightNing™ SmaI Cfr9I, TspMI, XmaI CCCGGG

                  5'...C C C ↓ G G G...3'
                  3'...G G G ↑ C C C...5'
                  blunt 25℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15574S LightNing™ SpeI AhlI,BcuI ACTAGT

                  5'...A ↓ C T A G T...3'
                  3'...T G A T C ↑ A...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: blocked.
                  EcoBI: blocked.

                  EG15575S LightNing™ SphI PaeI GCATGC

                  5'...G C A T G ↓ C...3'
                  3'...C ↑ G T A C G...5'
                  3’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: blocked.

                  EG15576S LightNing™ SspI AATATT

                  5'...A A T ↓ A T T...3'
                  3'...T T A ↑ T A A...5'
                  blunt 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15577S LightNing™ StuI Eco147I, PceI, SseBI AGGCCT

                  5'...A G G ↓ C C T...3'
                  3'...T C C ↑ G G A...5'
                  blunt 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: blocked.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15580S LightNing™ TaqI TCGA

                  5'...T ↓ C G A...3'
                  3'...A G C ↑ T...5'
                  5’ protruding ends 65℃ No (column purification)

                  Dam: blocked.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15581S LightNing™ XbaI TCTAGA

                  5'...T ↓ C T A G A...3'
                  3'...A G A T C ↑ T...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: blocked.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15582S LightNing™ XcmI CCANNNNNNNNNTGG

                  5'...C C A N N N N N ↓ N N N N T G G...3'
                  3'...G G T N N N N ↑ N N N N N A C C...5'
                  3’ protruding ends 37℃ 80℃,20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG15583S LightNing™ XhoI PaeR7I, Sfr274I, SlaI CTCGAG

                  5'...C ↓ T C G A G...3'
                  3'...G A G C T ↑ C...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG22507S BsmBI Esp3I CGTCTC(1/5)

                  5'...C G T C T C (N)?↓...3'
                  3'...G C A G A G (N)?↑...5'
                  5’ protruding ends 55℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: some blocked.
                  EcoKI: no effect.
                  EcoBI: impaired.

                  EG23503S BspQI SapI, LguI, PciSI GCTCTTC(1/4)

                  5'...G C T C T T C (N)?↓...3'
                  3'...C G A G A A G (N)?↑...5'
                  5’ protruding ends 50℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG21501S Sgel m?CNNG(9/13)

                  5'...m? C N N G(N)? ↓...3'
                  3'... G N N C(N)?? ↑...5'*
                  *SgeI 可識別與切割含有5-mC位點的DNA靶標序列,一條鏈或雙鏈甲基化均可被識別。
                  3’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effct.
                  CpG: no effect.
                  EcoKI: no effect.
                  EcoBI: no effect.

                  EG21504S Esp3I(BsmBI), ADCF BsmBI CGTCTC(1/5)

                  5'...C G T C T C (N)?...3'
                  3'...G C A G A G (N)?...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: blocked.
                  EcoKI: no effect.

                  EG22504S EcoRI, ADCF FunII, LraI, RsrI, SsoI GAATTC

                  5'...G ↓ A A T T C...3'
                  3'...C T T A A ↑ G...5'
                  5’ protruding ends 37℃ 80℃, 20 min

                  Dam: no effect.
                  Dcm: no effect.
                  CpG: impaired.
                  EcoKI: no effect.
                  EcoBI: no effect.

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